Selective Isolation and Characterization of Phytophthora infestans from Potatoes Using Rye Agar Media in India

Phytophthora infestans is a pathogen that causes late blight, major disease of potatoes. The isolation P. from infected potato plants using agar media has been challenging. This study investigated the use Rye A and B for tubers collected Nilgiris district Tamil Nadu during 2022. were evaluated hyphal growth, sporangial production, oospore formation, long-term storage infestans. Phenotypic diagnosis based on cultural morphological characteristics confirmed identity results by molecular identification test primers specific to Pathogenicity tests carried out assess virulence isolates. provides useful protocol selective characterization infestans, blight pathogen.